Part:BBa_K731520:Experience
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UNIQ6123693514a89647-partinfo-00000000-QINU UNIQ6123693514a89647-partinfo-00000001-QINU
Caracterization of BBa_K731520
iGEM Strasbourg team has access to fluorescence real-time plate reader (PheraStar Plus BM6 LABTECH), which is a good opportunity for obtaining high fidelity results on kinetics of inducible promoters. Our team chooses BBa_K731520 composite part for measured GFP expression rates under four different conditions : 0 μM, 20 μM, 100 μM and 500 μM of IPTG. Non-expressing GFP XL1 Blue bacteria were used as the negative control.
For detailed experimental protocol see iGEM Strasbourg 2019, (Lab work, Characterization ).
Using data from BBa_K731520 characterization we calculate the start point of GFP expression levels depending on IPTG concentration (Figure 2). These results could help the future teams to better choose experimental conditions using BBa_K731520 part.